thumb|250px|right|Location of Yoshino District in Nara Prefecture
is a district located in Nara Prefecture, Japan.
In 2020, the district had an estimated population of 37,086 and a density of 18.05 persons per km<sup>2</sup>. The total area is 2,055 km<sup>2</sup>.
On September 25, 2005, the villages of Ōtō and Nishiyoshino merged into the city of Gojō.
Towns and villages
- Higashiyoshino
- Kamikitayama
- Kawakami
- Kurotaki
- Nosegawa
- Ōyodo
- Shimoichi
- Shimokitayama
- Tenkawa
- Totsukawa
- Yoshino