VLB may refer to:
Science and technology
- VESA Local Bus, a local bus based on the Intel 80486 CPU
- Very large board, a large printed circuit board
- Vincaleukoblastine, or vinblastine, an antitumor alkaloid
Other uses
- VLB Berlin (Versuch- u. Lehranstalt für Brauerei in Berlin), a brewing school and trade organization in Berlin
- Volunteer Life Brigade, the forerunners of today Auxiliary Coastguard in the United Kingdom