U.S. Route 319 Business (US 319 Bus.) is a business route for US 319 that travels through the central portion of Moultrie. It is concurrent with SR 33 for its entire length.

US 319 Bus. begins at an intersection with US 319/SR 35 (Veterans Parkway) and SR 33 (Pavo Road) in the south-central part of Moultrie. US 319 Bus. and SR 33 travel concurrently as South Main Street to the north-northwest and immediately curve to the north-northeast. An intersection with 32nd Avenue SE leads to Colquitt Regional Medical Center. One block later, an intersection with 31st Avenue leads to the hospital, as well. Just past an intersection with the eastern terminus of Hugh Bannister Drive, they begin a curve back to the north-northwest. Just south of an intersection with the northern terminus of Lower Meigs Road, the highways curve back to the north-northeast. An intersection with 11th Avenue leads to the Jim Buck Goff Recreation Complex. At 4th Avenue, the northbound lanes of US 319 and SR 33 turn right and travel to the east, while the southbound lanes stay on South Main Street. South Main Street remains a two-way street until 2nd Avenue SE. One block later, they turn left onto 1st Street SE and continue their northern direction. On the southeastern corner of the intersection with 2nd Avenue SE lies the main office for Southwest Georgia Bank.

One block later, at 1st Avenue SE, they intersect the eastbound lanes of SR 37. Just north of this intersection, they pass the Colquitt County Courthouse. One block later, they intersect the westbound lanes of SR 37 (East Central Avenue). On the northeastern corner of this intersection lies the annex for the county courthouse. Here, 1st Street SE changes to 1st Street NE. At this same point, South Main Street changes back to a two-way street. At an intersection with 1st Avenue NE, 1st Street NE becomes a two-way street. On the southwestern corner of an intersection with 2nd Avenue NE lies the municipal building. On the northeaster corner lies the annex for the municipal building. Just north of 9th Avenue NE, they travel on a bridge over some railroad tracks of Norfolk Southern Railway (NS). North of Sylvester Drive, they have a roundabout with Sylvester Highway and the southbound lanes of US 319 Bus./SR 33. Just south of this roundabout is a sign for SR 33 south, even though it is actually still on Main Street. In fact, there is a directional sign in the roundabout that says that 1st Street NE is SR 33 south. The reunited lanes travel to the northeast. They cross over Okapilco Creek before they meet their northern terminus, an intersection with US 319/SR 35/SR 133 (Veterans Parkway/Tifton Highway). Here, SR 33 turns left onto East Bypass, concurrent with SR 133.

Between 2nd Avenue NE and 4th Avenue NW, they pass the U.S. post office for the city. Whereas the northbound lanes travel on a bridge over the NS line, the southbound lanes actually meet them at a railroad crossing. Just south of Sylvester Drive, the southbound lanes have an intersection with SR 111 (West Bypass NW). SR 111 does not meet the northbound lanes, or vice versa. Just north of Sylvester Drive, northbound Main Street has signage that indicates that it is northbound US 319 Bus./SR 33, although they are actually on 1st Street NE.

The entire length of US 319 Bus., and the portion of SR 33 concurrent with it, is part of the National Highway System, a system of routes determined to be the most important for the nation's economy, mobility, and defense.

  • Endpoints of U.S. Highway 319