Penola may refer to the following:
Places
;Antarctica
- Penola Island
- Penola Strait
;Australia
- Penola, South Australia, a town and locality
- Penola Conservation Park, a protected area in South Australia
- Hundred of Penola, a cadastral unit in South Australia
;United States
- Penola, Virginia, an unincorporated community in Virginia
Ships
- Penola, a sailing ship used for the British Graham Land expedition
- SS Penola, a steamship
Other
- Penola (fly), a genus of flies in the family Sphaeroceridae
- Penola Catholic College, a secondary school in Victoria, Australia