Mountain Brook may refer to:
Places in the United States
- Mountain Brook, Alabama, a city in southeastern Jefferson County, Alabama
- Mountain Brook School System, a public school system in Mountain Brook, Alabama
- Mountain Brook High School, a three-year public high school within the Mountain Brook School System
- Mountain Brook (New York), a tributary of the Little Delaware River near Bovina, New York
Livings things
- Duellmanohyla, or mountain brook frogs, a genus of frogs in the family Hylidae
- Ichthyomyzon greeleyi, also known as the mountain brook lamprey