Midland Line may refer to the following rail lines:
- Midland line, Perth, Western Australia
- Midland railway line, Western Australia
- Midland line (New Zealand)
- Midland line (New Haven), Massachusetts, United States
- Midland Main Line, between London St Pancras station, the East Midlands and Sheffield
See also
- Midland Mainline (train operating company), 1997-2006 company
- Midland Railway (disambiguation)
- Midland Railroad (disambiguation)