Middlesex is a historic county in southeast England.
Middlesex may also refer to:
Places
Australia
- Middlesex, Tasmania, a locality in Australia
- Middlesex, Western Australia, a locality of the Shire of Manjimup
Belize
- Middlesex, Belize, a village in Stann Creek District, Belize
Canada
- Middlesex County, Ontario
- Municipalities in Middlesex County:
- Middlesex Centre
- North Middlesex, Ontario
- Southwest Middlesex, Ontario
Jamaica
- Middlesex County, Jamaica
United Kingdom
- Middlesex (UK Parliament constituency)
United States
- Middlesex, New Jersey
- Middlesex, New York
- Middlesex, North Carolina
- Middlesex, Vermont
- Middlesex, Virginia
- Middlesex Township, Butler County, Pennsylvania
- Middlesex Township, Cumberland County, Pennsylvania
- Middlesex County, Connecticut
- Middlesex County, Massachusetts
- Middlesex County, New Jersey
- Middlesex County, Virginia
- West Middlesex, Pennsylvania, a borough in Mercer County
Literature
- Middlesex (novel), by Jeffrey Eugenides
Other
- Middlesex (1783 EIC ship), an East Indiaman of the British East India Company
- Middlesex Canal, Massachusetts
- Middlesex County Cricket Club, St John's Wood, London
- Middlesex Guildhall, Westminster, London
- Middlesex Middle School, Connecticut
- Middlesex Plat Historic District, listed on the National Register of Historic Places in Polk County, Iowa
- Middlesex School, Concord, Massachusetts
- Middlesex University, based in Hendon, London
- North Middlesex University Hospital, Edmonton, London
- West Middlesex University Hospital, Isleworth, London