Martinsburg may refer to:
Places
In the United States:
- Martinsburg, Indiana
- Martinsburg, Iowa
- Sandy Hook, Kentucky, originally incorporated as Martinsburg
- Martinsburg, Missouri
- Martinsburg, Ripley County, Missouri
- Martinsburg, Nebraska
- Martinsburg, New York
- Martinsburg, Ohio
- Martinsburg, Pennsylvania
- Martinsburg, West Virginia
In Germany:
- Martinsburg, Mainz, a fortress which was demolished in 1809 (see Electoral Palace Mainz)