The following highways are numbered 401:

Canada

  • British Columbia Highway 401 (former)
  • Manitoba Provincial Road 401
  • 20px Ontario Highway 401

Costa Rica

  • 25px National Route 401

Cuba

  • 28x28px Road to El Fe (4–401)

Japan

  • 20px Japan National Route 401

Norway

  • 30px Norwegian County Road 401

Thailand

  • 25px Thailand Route 401

United States

  • 25px U.S. Route 401
  • Florida:
  • 25px Florida State Road 401
  • 25px County Road 401 (Brevard County, Florida)
  • 25px Georgia State Route 401 (unsigned designation for Interstate 75)
  • 25px Kentucky Route 401
  • 25px Louisiana Highway 401
  • 25px Nevada State Route 401
  • New York:
  • 25px New York State Route 401 (former)
  • 25px County Route 401 (Albany County, New York)
  • 25px North Carolina Highway 401 (former)
  • 25px Pennsylvania Route 401
  • 40px|link=|alt= 40px|link=|alt= 25px|link=|alt= Pennsylvania Route 401 Alternate Truck
  • 25px Puerto Rico Highway 401
  • 25px Rhode Island Route 401
  • 25px Virginia State Route 401
  • 25px Washington State Route 401