thumb|right|Río Negro Province, situation

List of the most important cities, towns and villages in the Río Negro Province in Argentina, with their municipal population ().

  • Allen (26,083)
  • Campo Grande (4,571)
  • Colonia Catriel (15,169)
  • Cervantes (5,173)
  • Chichinales (4,060)
  • Chimpay (3,905)
  • Choele Choel (10,642)
  • Cinco Saltos (19,819)
  • Cipolletti (75,078)
  • Comallo (1,306)
  • Contralmirante Cordero (2,782)
  • Coronel Belisle (1,841)
  • Darwin (1,052)
  • Dina Huapi (2,243)
  • El Bolsón, Río Negro (15,537)
  • General Conesa (5,595)
  • General Fernández Oro (6,813)
  • General Roca (78,275)
  • Ingeniero Jacobacci (5,785)
  • Ingeniero Luis A. Huergo (6,483)
  • Lamarque (7,819)
  • Los Menucos (2,689)
  • Luis Beltrán (6,401)
  • Mainqué (2,658)
  • Maquinchao (2,195)
  • Pilcaniyeu (1,467)
  • Pomona (987)
  • Río Colorado (13,675)

thumb|right|Villa Regina

  • San Antonio Oeste (16,966)
  • Las Grutas (approx. 2,500)
  • San Carlos de Bariloche (93,101)
  • Sierras Coloradas (1,374)
  • Sierra Grande (6,978)
  • Valcheta (3,596)
  • Viedma (47,437)
  • Balneario El Cóndor (approx. 1,300)
  • Villa Regina (31,209)