This is a list of railroads operating in the U.S. state of Florida.
Current railroads
Common freight carriers
- Alabama and Gulf Coast Railway (AGR) (GWI)
- AN Railway (AN) (GWI)
- Bay Line Railroad (BAYL) (GWI)
- CSX Transportation (CSXT)
- First Coast Railroad (FCRD) (GWI)
- Florida Central Railroad (FCEN)
- Florida East Coast Railway (FEC)
- Florida Gulf & Atlantic Railroad (FGA)
- Florida Midland Railroad (FMID)
- Florida Northern Railroad (FNOR)
- Georgia and Florida Railway (GFRR)
- Jacksonville Port Terminal Railroad (JXPT) (Watco)
- Norfolk Southern Railway (NS) through subsidiary Georgia Southern and Florida Railway (GSF)
- Seminole Gulf Railway (SGLR)
- South Central Florida Express, Inc. (SCXF)
Private freight carriers
- CF Industries (CFIZ)
- Conrad Yelvington Distributors, Inc. (CNYX)
- The Mosaic Company (IMCX)
- Orlando Utilities Commission (OUCX)
- Perdue Farms (PFMX)
- Port Manatee Railroad (MAUP)
- Port of Palm Beach District (PPBD)
- Port of Panama City (PPC)
- United States Sugar Corporation (USSC)
Passenger carriers
- Amtrak (AMTK)
- Brightline (BLFX)
- Florida Railroad Museum (FGCX)
- Gold Coast Railroad Museum (GCOX)
- Jacksonville Skyway
- Kirby Family Farm Train
- Miami Metromover and Miami Metrorail
- Seminole Gulf Railway (SGLR)
- Serengeti Express (located in Busch Gardens Tampa)
- Sugar Express (CHOX)
- SunRail (CFRC)
- Tradewinds & Atlantic Railroad
- TECO Line Streetcar
- Tri-Rail (TRCX)
- Walt Disney World
- Walt Disney World Monorail and Walt Disney World Railroad
- Wildlife Express Train
Private carriers
- Clay Springs and Apopka Railroad<!--?-->
- Ellaville, West Lake and Jennings Railroad
- Florida and Georgia Railway<!--?-->
- Peace River Phosphate Company
- Pensacola and Andalusia Railroad
- Starke and New River Railroad
- Union Cypress Company
- Winston Lumber Company
Never built
- Apopka and Atlantic Railroad
- Choctawhatchee and Northern Railroad
- Glen Cove Springs and Fort Fanning Railroad
Defunct railroads
<!--"Municipal Docks and Terminals"? Ocala and Silver Springs? Sebastian and Cincinnatus Farms? Pensacola and Northern Alabama? Escambia? Florida and Alabama? I know the Central of Georgia entered, presumably through the Chattahoochee and Gulf Railroad, but when? Live Oak, Luraville and Deadman's Bay Railroad?-->
{| class="wikitable sortable"
!Name
!Mark
!System
!From
!To
!Successor
!Notes
|-
|Alabama and Florida Railroad (1853–1869)
|
|L&N
|1853
|1869
|Pensacola and Louisville Railroad
|-
|Alabama and Florida Railroad (1898–1900)
|
|L&N
|1898
|1900
|Louisville and Nashville Railroad
|-
|Alabama and Florida Railroad (1936–1941)
|
|
|1936
|1941
|Ceased operations in 1941
|-
|Alabama and Florida Railroad (1986–1992)
|AFLR
|
|1986
|1992
|Alabama and Florida Railway
|-
|Alabama and Florida Railway
|AF
|
|1992
|2011
|Ceased operations in 2011
|-
|Alabama and Western Florida Railroad
|
|
|1926
|1939<!--?-->
|
|-
|Alabama, Florida and Gulf Railroad
|
|
|1917
|1936
|Alabama and Florida Railroad (1936–1941)
|-
|Alabama, Florida and Southern Railroad
|
|
|1910
|1917
|Alabama, Florida and Gulf Railroad
|-
|Alafia, Manatee, and Gulf Coast Railroad
|
|SAL
|1897
|1906
|Charlotte Harbor and Northern Railway
|-
|Andalusia, Florida and Gulf Railway
|
|
|1919
|1925
|N/A
|
|-
|Florida, Alabama and Gulf Railroad
|
|
|before 1911
|1919
|Andalusia, Florida and Gulf Railway
|
