Interstate 140 may refer to:
- Interstate 140 (North Carolina), a spur route near Wilmington, North Carolina
- Interstate 240 (North Carolina), a bypass of Asheville, North Carolina, formerly known as I-140
- Interstate 140 (North Carolina 1999), a cancelled proposed spur between Sanford and Raleigh, North Carolina
- Interstate 140 (Tennessee), a spur route in Knoxville, Tennessee