I-140 may refer to:

  • Interstate 140 (North Carolina), a bypass of Wilmington, North Carolina
  • Interstate 140 (Tennessee), a spur route in Knoxville, Tennessee
  • Form I-140, Immigrant Petition for Alien Worker, a form that needs to be filed as part of the application process for an employment-based visa for the United States
  • Iodine-140 (I-140 or <sup>140</sup>I), an isotope of iodine