Holtzendorff or von Holtzendorff can refer to:
- (), a Brandenburgian (Old Märkisch) noble family, an Uradel der Uckermark, later Silesia, Ostpreußen, Pommerania, Royal Saxony, and Mecklenburg.
- Georg Holtzendorff, 19th-century German painter
- Henning von Holtzendorff (18531919), a German admiral
- Joachim Wilhelm Franz Philipp von Holtzendorff (18291889), a German jurist