Gristle may refer to:
- In butchery and gastronomy, various tough and inedible meat tissues
- Cartilage, connective tissue
- Fascia, connective tissue sheaths
- Epimysium, fibrous tissue surrounding many muscles
- Throbbing Gristle, an English avant-garde music and visual arts group
- Gristle (G.I. Joe), a fictional villain in the G.I. Joe universe
- Puppy Gristle, a 2002 album by Skinny Puppy
- The House of Gristle, a 1994 British television series