German submarine U-464 was a Type XIV supply and replenishment U-boat ("Milchkuh") of Nazi Germany's Kriegsmarine during World War II.
Her keel was laid down on 18 March 1941, by Deutsche Werke in Kiel as yard number 295. She was launched on 20 December 1941 and commissioned on 30 April 1942 with Kapitänleutnant Otto Harms in command. He remained in charge for her entire career.