Fort Defiance may refer to:

;Canada

  • Fort Defiance (British Columbia), winter quarters for American Captain Robert Gray

;United States

  • Fort Defiance, Arizona, an unincorporated community
  • Fort Defiance (California), formerly Roop's Fort, located in Susanville
  • Fort Morris, Georgia, known as Fort Defiance during the War of 1812
  • Fort Defiance (Illinois), a Civil War post commanded by General Ulysses S. Grant, site of Fort Defiance Park
  • Fort Defiance State Park near Estherville, Iowa
  • Fort Defiance (Maryland), a War of 1812 fort on the Elk River
  • Fort Defiance (Massachusetts), a 19th-century fort in Gloucester
  • Fort Defiance (Brooklyn), a fort in the neighborhood of Red Hook, Brooklyn, New York during the American Revolution
  • Fort Defiance (Lenoir, North Carolina), former plantation home of General William Lenoir
  • Fort Defiance (Ohio), in present-day Defiance, Ohio
  • Gordon Stockade, South Dakota, initially called Fort Defiance
  • Fort Defiance, Tennessee, in Clarksville TN, later renamed Fort Bruce
  • Fort Defiance, briefly the name of the Presidio La Bahía in Goliad, Texas during the Texas Revolution
  • Fort Defiance (Vermont), a fort in Vermont
  • Fort Defiance, Virginia, an unincorporated community
  • Fort Defiance (Wisconsin), a fort during the Black Hawk War

;In film

  • Fort Defiance (film), a 1951 Western directed by John Rawlins