Cheselbourne (sometimes spelled Chesilborne or Cheselborne) is a village and civil parish in Dorset, England, situated in the Dorset Downs, north-east of Dorchester. The parish is at an altitude of 75 to 245 metres (approximately 250 to 800 feet) and covers an area of ; the underlying geology is chalk. In the 2011 census the parish had a population of 296. and a canonical sundial.
thumb|left|Canonical sundial on the parish church
In 1086, in the Domesday Book Cheselbourne was recorded as Ceseburne; it had 36 households, of meadow and one mill. It was in the hundred of Hilton and the lord and tenant-in-chief was Shaftesbury Abbey.
Cheselbourne used to be the site of a tradition known as "Treading in the Wheat", in which young women from the village would walk the fields on Palm Sunday, dressed in white.