Charles Jules Henri Nicolle (21 September 1866 – 28 February 1936) He had two other siblings – his older brother, Maurice Nicolle (a medical microbiologist, professor at the Pasteur Institute in Paris, and Director of the Bacteriological Institute of Constantinople), and his younger brother, Marcel Nicolle (an art critic).
Nicole later married Alice Avice in 1895 and had two children, Marcelle (b. 1896) and Pierre (b. 1898), both of whom also went on to enter the medical field. At this point he returned to Rouen, as a member of the Medical Faculty until 1896 and then as Director of the Bacteriological Laboratory from 1896 to 1902. He was still director of the Institute when he died in 1936. However, under Nicolle’s guidance over the next 33 years, the 'sister' Institute in Tunis quickly became an international centre of its own for the production of vaccines used against infectious diseases and for medical research. Within 10 days, the second chimpanzee had typhus as well. After repeating his experiment, he was sure of it: lice were the carriers.