Betis can refer to:
- The Latin name of the Guadalquivir river, which with its surrounding area constituted a province of the Roman Empire
- Real Betis, a football team based in Seville
- Betis Deportivo Balompié, the reserve team of Real Betis
- Betis CF, a football team based in Valladolid
- A district in the Philippine municipality of Guagua, Pampanga
- Betis Church, a Roman Catholic church in Guagua, Philippines
- The Indonesian and Malay word for calf