Barnesville may refer to:
In Canada:
- Barnesville, New Brunswick
In the USA:
- Barnesville, Alabama
- Barnesville, Georgia
- Barnesville, Kansas
- Barnesville's Post
- Barnesville, Maryland
- Barnesville (MARC station)
- Barnesville, Minnesota
- Barnesville, Clinton County, Missouri
- Barnesville, Macon County, Missouri
- Barnesville, North Carolina
- Barnesville, Ohio
- Barnesville Baltimore and Ohio Railroad Depot
- Barnesville, Pennsylvania
- Barnesville Township, Clay County, Minnesota