Ambrosio may refer to:
People
- Alessandra Ambrosio (born 1981), a Brazilian model
- Arturo Ambrosio (1870–1960), an Italian film producer
- Fabrisia Ambrosio, Brazilian-born physical therapist and academic
- Franco Ambrosio (1932–2009), an Italian businessman
- Frank Ambrosio (born 1949), American philosopher
- Gabriele Ambrosio (1844–1918), Italian sculptor
- Giovanni Ambrosio (1420–1484), Italian dancer and writer on dance (Guglielmo Ebreo da Pesaro)
- Luigi Ambrosio (born 1963), an Italian mathematician
- Marco Ambrosio (born 1973), Italian former footballer
- Rodolfo Ambrosio (born 1961), Argentine rugby union coach
- Sandra Ambrosio (born 1963), Argentine former cyclist
- Thomas Ambrosio (born 1971), American political scientist
- Valentino Ambrosio (born 2000), American football player
- Vittorio Ambrosio (1879–1958), an Italian general from World War I and World War II
Other
- Ambrosio (horse), a racehorse
- Ambrosio Film, an Italian film production company that existed from 1906 to 1924
See also
- D'Ambrosio
- Ambrosiano (disambiguation)