ACSL may refer to:
- American Computer Science League, a computer science competition primarily among US high school students
- Advanced Continuous Simulation Language, a computer language
- ANSI/ISO C Specification Language, a formal specification language for C programs
- Association of School and College Leaders, a British professional association
- Human genes encoding Acyl-CoA synthetase long-chain enzymes:
- ACSL1
- ACSL3
- ACSL4
- ACSL5
- ACSL6
- SLC27A2
- The Anti-Capitalist Software License