The 2001 Major League Soccer SuperDraft was held on February 5, 2001, at the Signature Grand in Davie, Florida.

Player selection

;Key

{| class="wikitable"

| style="background:#FFCC00"|*

| Denotes a player contracted under the Project-40 program

|-

| style="background:#CCFF99"|§

| Denotes a player who won the MLS Rookie of the Year

|-

| style="background:#CFECEC"| ^

| Denotes player who has been selected to an MLS All-Star Game

|-

| style="background: #FBCEB1"| †

| Denotes player who has been selected for an MLS Best XI team

|-

| style="background:#FFFF99"| ~

|Denotes a player who won the MLS MVP

|-

|}

Round 1

thumb|[[D.C. United selected Ryan Nelsen 4th overall. Nelsen is a 2x MLS Best XI selection. He earned 49 caps for the New Zealand Men's National Team and was selected to the 2010 FIFA World Cup squad.]]

thumb|The [[Sporting Kansas City|Kansas City Wizards selected José Burciaga Jr. 12th overall. He is a 2006 MLS Best XI selection and won the 2008 MLS Humanitarian of the Year Award.]]

{| class="wikitable sortable"

|-

!width=7%| Pick # !!width=27%| MLS team !!width=24%| Player !!width=10%| Position !!width=33%| Affiliation

|-

! 1

| San Jose Earthquakes || style="background:#FFCC00"|Chris Carrieri* || F || University of North Carolina

|-

! 2

| Tampa Bay Mutiny